Okay, thank you.
Then is there any difference between a FAT32 / NTFS / WBFS partition? Or any difference between ISO or WBFS files?
Regarding speed no, it should be the same. In every case a direct sector read is done. The way it is implemented there is no filesystem and file format overhead - only a list of sectors on the drive. The only case where there could be a difference would be a heavy-heavy fragmented filesystem (wbfs can be fragmented too, but the blocks are larger), but even if it's fragmented it should be not be noticeable - consider that even if the drive is heavily fragmented it would still be way faster than a dvd. I think I made some performance testing a long long time ago and posted it somewhere here on gbatemp, but i guess it would be hard to find. Bottom line is performance should be the same, it's more a question of convenience and preference.